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paav hsyn egfp vector  (Addgene inc)


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    Addgene inc paav hsyn egfp vector
    Paav Hsyn Egfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 375 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/paav+hsyn+egfp+vector/pAAV-hSyn-EGFP+(Plasmid+%2350465)/pm41912102-306-1-4
    Average 96 stars, based on 375 article reviews
    paav hsyn egfp vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cloning:

    Article Title: miR-329 and miR-495-mediated Prr7 downregulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Supplemental Methods. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3’ UTR of eGFP in pAAV-hSyn-EGFP vector (Addgene Plasmid #114213) using BsrGI and HindIII sites, as described previously ( ). .. Control and Prr7 shRNA vectors were constructed using the pSUPER RNAi System (OligoEngine).

    Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Table S1. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3′ UTR of eGFP in the pAAV-hSyn-EGFP vector (Plasmid #114213; Addgene) using BsrGI and HindIII sites, as described previously ( ). ..

    Over Expression:

    Article Title: miR-329 and miR-495-mediated Prr7 downregulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Supplemental Methods. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3’ UTR of eGFP in pAAV-hSyn-EGFP vector (Addgene Plasmid #114213) using BsrGI and HindIII sites, as described previously ( ). .. Control and Prr7 shRNA vectors were constructed using the pSUPER RNAi System (OligoEngine).

    Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Table S1. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3′ UTR of eGFP in the pAAV-hSyn-EGFP vector (Plasmid #114213; Addgene) using BsrGI and HindIII sites, as described previously ( ). ..

    Generated:

    Article Title: miR-329 and miR-495-mediated Prr7 downregulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Supplemental Methods. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3’ UTR of eGFP in pAAV-hSyn-EGFP vector (Addgene Plasmid #114213) using BsrGI and HindIII sites, as described previously ( ). .. Control and Prr7 shRNA vectors were constructed using the pSUPER RNAi System (OligoEngine).

    Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Table S1. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3′ UTR of eGFP in the pAAV-hSyn-EGFP vector (Plasmid #114213; Addgene) using BsrGI and HindIII sites, as described previously ( ). ..

    Plasmid Preparation:

    Article Title: miR-329 and miR-495-mediated Prr7 downregulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Supplemental Methods. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3’ UTR of eGFP in pAAV-hSyn-EGFP vector (Addgene Plasmid #114213) using BsrGI and HindIII sites, as described previously ( ). .. Control and Prr7 shRNA vectors were constructed using the pSUPER RNAi System (OligoEngine).

    Article Title: miR-329– and miR-495–mediated Prr7 down-regulation is required for homeostatic synaptic depression in rat hippocampal neurons
    Article Snippet: .. All primer sequences used for cloning are indicated in Table S1. miR-30a-chimeric hairpins for miR-329 and miR-495 stable overexpression were generated via polynucleotide cloning into the 3′ UTR of eGFP in the pAAV-hSyn-EGFP vector (Plasmid #114213; Addgene) using BsrGI and HindIII sites, as described previously ( ). ..

    Article Title: Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke
    Article Snippet: .. Surgical Procedures for Virus Inoculation. pAAV-hGFAP-G-CaMP7 (a gift from Masanori Matsuzaki, University of Tokyo, Tokyo; ref. 62 ) was synthesized by modifying the pAAV-hSyn-EGFP vector (Addgene plasmid #50465, a gift from Bryan Roth, University of North Carolina School of Medicine, Chapel Hill, NC) with a human GFAP promoter ( 63 ) and G-CaMP7 cDNA. ..

    Article Title: Genetically encoded sensors for analysing neurotransmission among synaptically-connected neurons
    Article Snippet: .. The pAAV-hSyn-EGFP vector was a gift from Dr Bryan Roth (Addgene plasmid #50465). .. TagBFP-P2A-Pre-Sphere-SF-iGluSnFR and mCherry-P2A-Post-Sphere-SF-iGluSnFR sequences were inserted into the pAAV-hSyn-EGFP Vector by replacing the EGFP using Infusion (Takara Bio).

    Article Title: Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke
    Article Snippet: .. pAAV-hGFAP-G-CaMP7 (a gift from Masanori Matsuzaki, University of Tokyo, Tokyo; ref. 62 ) was synthesized by modifying the pAAV-hSyn-EGFP vector (Addgene plasmid #50465, a gift from Bryan Roth, University of North Carolina School of Medicine, Chapel Hill, NC) with a human GFAP promoter ( 63 ) and G-CaMP7 cDNA. ..

    Polymerase Chain Reaction:

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
    Article Snippet: Then, both fragments were ligated into the pAAV-hSyn-hM3D(Gq)-mCherry vector and pAAV-hSyn-hM4D(Gi)-mCherry vector (Addgene, #50474 and #50475) using In-Fusion (Takara, #639648). .. For pAAV-hSyn-Mito-DsRed, the coding sequences of Mito-DsRed were PCR-amplified from the pDsRed2-Mito vector (TakaRa, #632421), and the fragments were ligated into the pAAV-hSyn-EGFP vector (Addgene, #50465) using In-Fusion. .. For pAAV-hSyn-synaptophysin-mCherry, the coding sequences of synaptophysin and mCherry were PCR-amplified from a mouse cDNA library and pAAV-CaMKIIa-hM3D(Gq)-mCherry (Addgene, #50476), respectively.

    Article Title: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia.
    Article Snippet: Then, both fragments were ligated into the pAAV-hSyn-hM3D(Gq)-mCherry vector and pAAV-hSyn-hM4D(Gi)-mCherry vector (Addgene, #50474 and #50475) using In-Fusion (Takara, #639648). .. For pAAV-hSyn-MitoDsRed, the coding sequences of Mito-DsRed were PCR-amplified from the pDsRed2-Mito vector (TakaRa, #632421), and the fragments were ligated into the pAAV-hSyn-EGFP vector (Addgene, #50465) using In-Fusion. .. For pAAV-hSyn-synaptophysin-mCherry, the coding sequences of synaptophysin and mCherry were PCR-amplified from a mouse cDNA library and pAAV-CaMKIIa-hM3D(Gq)-mCherry (Addgene, #50476), respectively.

    Virus:

    Article Title: Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke
    Article Snippet: .. Surgical Procedures for Virus Inoculation. pAAV-hGFAP-G-CaMP7 (a gift from Masanori Matsuzaki, University of Tokyo, Tokyo; ref. 62 ) was synthesized by modifying the pAAV-hSyn-EGFP vector (Addgene plasmid #50465, a gift from Bryan Roth, University of North Carolina School of Medicine, Chapel Hill, NC) with a human GFAP promoter ( 63 ) and G-CaMP7 cDNA. ..

    Synthesized:

    Article Title: Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke
    Article Snippet: .. Surgical Procedures for Virus Inoculation. pAAV-hGFAP-G-CaMP7 (a gift from Masanori Matsuzaki, University of Tokyo, Tokyo; ref. 62 ) was synthesized by modifying the pAAV-hSyn-EGFP vector (Addgene plasmid #50465, a gift from Bryan Roth, University of North Carolina School of Medicine, Chapel Hill, NC) with a human GFAP promoter ( 63 ) and G-CaMP7 cDNA. ..

    Article Title: Adrenergic receptor antagonism induces neuroprotection and facilitates recovery from acute ischemic stroke
    Article Snippet: .. pAAV-hGFAP-G-CaMP7 (a gift from Masanori Matsuzaki, University of Tokyo, Tokyo; ref. 62 ) was synthesized by modifying the pAAV-hSyn-EGFP vector (Addgene plasmid #50465, a gift from Bryan Roth, University of North Carolina School of Medicine, Chapel Hill, NC) with a human GFAP promoter ( 63 ) and G-CaMP7 cDNA. ..

    other:

    Article Title: Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β-TFEB signaling axis.
    Article Snippet: Subsequently, they were washed twice and filtered prior to loading into the separation column (LS column) attached to a magnetic stand (all from Miltenyi Biotec).



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    Addgene inc paav hsyn egfp wpre vector
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    Regulating O-GlcNAcylation affects dimeric α-syn formation. ( A ) schematic illustration of the procedure for detecting O-GlcNAcylated α-syn (Oα-syn) in PFFs-seeded SH-SY5Y cells and mouse brain treated with OSMI-1 or TMG using the click-iT™ O-GlcNAc enzyme labeling system. ( B ) immunoblot analysis of O-GlcNAcylated α-syn (Oα-syn) in PFFs-seeded SH-SY5Y cells. Ponceau S staining serves as a loading control. Arrowhead indicates the Oα-syn bands that shifted from a higher molecular weight (HMW) of ~ 35 kDa (dimeric α-syn) to ~ 55 kDa in the presence of GalT1 (Y289L). ( C ) quantification of α-syn and the proportion (%) of Oα-syn in the dimeric and total α-syn in the cells treated with OSMI-1 or TMG ( n = 6). ( D ) examination of Oα-syn in SH-SY5Y cells transfected with <t>α-syn-eGFP</t> plasmid. ( E ) representative immunoblot images of Oα-syn in the 6 months post-injection of PFFs. ( F ) quantification of the Oα-syn in the brain of the mice 6 months post-injection of PFFs. Oα-syn levels normalized to GAPDH ( n = 3). ( G - H ) immunoblot analysis of immunoprecipitated α-syn in SH-SY5Y cells transfected with α-syn-eGFP plasmids for 36 h to 72 h ( G ) or exposed to OSMI-1 or TMG ( H ) using a pan O-GlcNAc antibody (RL2). ( I ) sWGA pull-down assay of α-syn in the brains of AAV/PFFs mice treated with OSMI-1 or TMG. ( J ) quantification of α-syn from the input and sWGA pull-down fractions. α-syn and Oα-syn were normalized to GAPDH (input) or the densitometric gray value (IP). Data are shown as means ± SEM. * p < 0.05, ** p < 0.01, * p < 0.001, and ** p < 0.0001 by one-way ANOVA with Tukey’s post-hoc test
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    Image Search Results


    Regulating O-GlcNAcylation affects dimeric α-syn formation. ( A ) schematic illustration of the procedure for detecting O-GlcNAcylated α-syn (Oα-syn) in PFFs-seeded SH-SY5Y cells and mouse brain treated with OSMI-1 or TMG using the click-iT™ O-GlcNAc enzyme labeling system. ( B ) immunoblot analysis of O-GlcNAcylated α-syn (Oα-syn) in PFFs-seeded SH-SY5Y cells. Ponceau S staining serves as a loading control. Arrowhead indicates the Oα-syn bands that shifted from a higher molecular weight (HMW) of ~ 35 kDa (dimeric α-syn) to ~ 55 kDa in the presence of GalT1 (Y289L). ( C ) quantification of α-syn and the proportion (%) of Oα-syn in the dimeric and total α-syn in the cells treated with OSMI-1 or TMG ( n = 6). ( D ) examination of Oα-syn in SH-SY5Y cells transfected with α-syn-eGFP plasmid. ( E ) representative immunoblot images of Oα-syn in the 6 months post-injection of PFFs. ( F ) quantification of the Oα-syn in the brain of the mice 6 months post-injection of PFFs. Oα-syn levels normalized to GAPDH ( n = 3). ( G - H ) immunoblot analysis of immunoprecipitated α-syn in SH-SY5Y cells transfected with α-syn-eGFP plasmids for 36 h to 72 h ( G ) or exposed to OSMI-1 or TMG ( H ) using a pan O-GlcNAc antibody (RL2). ( I ) sWGA pull-down assay of α-syn in the brains of AAV/PFFs mice treated with OSMI-1 or TMG. ( J ) quantification of α-syn from the input and sWGA pull-down fractions. α-syn and Oα-syn were normalized to GAPDH (input) or the densitometric gray value (IP). Data are shown as means ± SEM. * p < 0.05, ** p < 0.01, * p < 0.001, and ** p < 0.0001 by one-way ANOVA with Tukey’s post-hoc test

    Journal: Molecular Neurodegeneration

    Article Title: Modulation of O-GlcNAc cycling influences α-synuclein amplification, degradation, and associated neuroinflammatory pathology

    doi: 10.1186/s13024-025-00904-2

    Figure Lengend Snippet: Regulating O-GlcNAcylation affects dimeric α-syn formation. ( A ) schematic illustration of the procedure for detecting O-GlcNAcylated α-syn (Oα-syn) in PFFs-seeded SH-SY5Y cells and mouse brain treated with OSMI-1 or TMG using the click-iT™ O-GlcNAc enzyme labeling system. ( B ) immunoblot analysis of O-GlcNAcylated α-syn (Oα-syn) in PFFs-seeded SH-SY5Y cells. Ponceau S staining serves as a loading control. Arrowhead indicates the Oα-syn bands that shifted from a higher molecular weight (HMW) of ~ 35 kDa (dimeric α-syn) to ~ 55 kDa in the presence of GalT1 (Y289L). ( C ) quantification of α-syn and the proportion (%) of Oα-syn in the dimeric and total α-syn in the cells treated with OSMI-1 or TMG ( n = 6). ( D ) examination of Oα-syn in SH-SY5Y cells transfected with α-syn-eGFP plasmid. ( E ) representative immunoblot images of Oα-syn in the 6 months post-injection of PFFs. ( F ) quantification of the Oα-syn in the brain of the mice 6 months post-injection of PFFs. Oα-syn levels normalized to GAPDH ( n = 3). ( G - H ) immunoblot analysis of immunoprecipitated α-syn in SH-SY5Y cells transfected with α-syn-eGFP plasmids for 36 h to 72 h ( G ) or exposed to OSMI-1 or TMG ( H ) using a pan O-GlcNAc antibody (RL2). ( I ) sWGA pull-down assay of α-syn in the brains of AAV/PFFs mice treated with OSMI-1 or TMG. ( J ) quantification of α-syn from the input and sWGA pull-down fractions. α-syn and Oα-syn were normalized to GAPDH (input) or the densitometric gray value (IP). Data are shown as means ± SEM. * p < 0.05, ** p < 0.01, * p < 0.001, and ** p < 0.0001 by one-way ANOVA with Tukey’s post-hoc test

    Article Snippet: The resulting fragment was inserted into the backbone of the pAAV-hsyn- eGFP -WPRE vector (Addgene item no. #50465).

    Techniques: Labeling, Western Blot, Staining, Control, Molecular Weight, Transfection, Plasmid Preparation, Injection, Immunoprecipitation, Pull Down Assay